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In some strains of Saccharomyces cerevisiae the mitochondrial gene coding for 21S rRNA is interrupted by an 21x of bp. This intron contains a reading frame for amino acids: In order to check whether expression of this URF is required for proper splicing of precursors to 21S rRNA, the precision of RNA splicing was analysed in a petite mutant, where no mitochondrial protein synthesis is possible anymore.
We have devised a new assay to monitor the precision of the splicing event.
The method is of dmb application, provided that the sequence of the splice boundaries is known. Resistance to digestion will only be observed if the correct splice-junction is made.
In RNA blotting experiments, using an intron specific hybridisation probe, the same intermediates in splicing are found both in wild type and petite mutant. Finally the synthetic oligonucleotide hybridises to petite 21S rRNA and its thermal dissociation behaviour is indistinguishable from a hybrid formed with wildtype 21S rRNA. Most users should sign in with their email address.
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Abstract In some strains of Saccharomyces cerevisiae the mitochondrial gene coding for 21S rRNA is interrupted by an intron of bp. You do not currently have access to this article. Add comment Close comment form modal. I agree to the terms and conditions.
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Email alerts New issue alert. Receive exclusive offers and updates from Oxford Academic. More on this topic Comparison of intron-containing and intron-lacking human 21a elucidates putative exonic splicing enhancers.
CNB-DBM-21S Dome Cam(600TVL)(f=3.8mm)
Splicing of many human genes involves sites embedded within introns. Design and evaluation of locked nucleic acid-based splice-switching oligonucleotides in vitro. Related articles in Web of Science Google Scholar. Related articles in PubMed Genome-wide 21d study of degenerative mitral valve disease in Maltese dogs. Factor cooperation for chromosome discrimination in Drosophila.
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